Lufthansa Group pilots, represented by the Vereinigung Cockpit (VC) union, will strike for six hours Friday, which will affect short- and medium-haul flights from Frankfurt Airport.
More than 200 flights and 25,000 passengers will be affected by the strike action.
VC said earlier that talks with Lufthansa management had failed to reach an agreement over its early retirement scheme.
Lufthansa low-cost carrier subsidiary Germanwings had to cancel 116 out of 164 flights last week due to a strike over transitional benefits. The strike affected 15,000 passengers.